From a ribosomal RNA view. It appears we (humans) are similar this way to a slime mold, which is fascinating to me. Gram positives are shown. The phyla (Proteobacteria, Chlamydias, Spirochetes, Cyanobacteria, and others) are Gram-negative.
Credit: Carl Woese, Microbiologist
Taste the wild
Lick the wind
Like something they never saw before
Their jaws dropping to the floor
Steel made of soul and sin
Rebels born without a care
(And the day he listens)
Only to fly where eagles dare
And the night she whispers
Ride the wind
Never coming back until I touch the midnight sun
(Ride the wind)
(Never coming back again)
Ride the wind
Never coming back until I touch the midnight sun
Painted flesh
Loyalty
Humble pride
Just as far as the eye can see
Stories told
Two old friends
Of battle scars and lonely bars
And nights the rain wouldn’t end
Here’s to withered eyes wearing gypsy smiles
(And the day he listens)
Here’s to lovely ladies and a million miles
And the night she whispers
Poison lyrics. “Ride the wind”
I used some of these arrangements for a truss, from Jon Peters youtube channel. Always experimenting, this is for a small cabin, going to use plywood gusset plates AND steel gusset plates.
I am using 24 inches on center, for each truss to hold this cabin roof.
Building Roof truss systems for shed, barn, or a tiny house by Jon Peters
Noticed a few of these in Riverside / palm springs area . Now I know what they do. They capture the stickers you have on your car, among other things. Mass surveillance has now started nationwide. Good news is that many cities are cancelling the contracts.
DeFlock Maps | ALPR Camera Map & Privacy Routes
It seems if this service and devices were more open to exactly what they were doing, how they did it, and was accessible locally ONLY to the city who installed the devices, perhaps it would not be so disgusting and difficult for people. Cameras like this that upload to the cloud are security problems waiting to happen. Stalking? Crime prevention? Investigators and police will try to justify recording every person in every square foot of your city.
Where is your local tax money going? Better to pay police officers, or pay for technology like this?
I have puppies, 6 if you want some call me 951 201 9999. Remove 9999 add 1091.
Above drawing shows microscope using immersion microscopy using only one slide, with instead of oil immersion, the lens of the microscope is immersed in the specimen solution.
Yes, the microscope lens is immersed not in oil, but in the solution itself with the specimen, the specimen you are attempting to view with the microscope. Read up on immersion microscopy to understand the differences and why these images are improved. Below is example of red blood cells I took using immersion photograph using a microscope. Note many more blurred objects can be seen, circled in yellow as the specimen depth of field and optical quality is a different versus having the air refraction.
One advantage is the the specimens are not compressed between two slides. This might be good for viewing live cells or live organisms.
A disadvantage, live organisms would move quite a bit and be difficult to track in the microscope.
Ideally you would have a solution like oil that has the same index of refraction of the glass slides. I assume the water solution has a different index of refraction than the glass.
Using the gel electrophoresis device, I found the simple concept not so simple. This was using Agarose gel analysis. Agarose with a direct current, is the most commonly used method for analyzing DNA fragments between 0.1 and 25 kb, while pulse-field gel electrophoresis enables analysis of DNA fragments up to 10,000 kb. Our experiment used 100 volts Dc.
My questions. If the DNA simply moved towards one of the charged sides, why doesn’t the DNA move all the way through the gel? Large DNA molecules do not work in electrophoresis depending on the gel type. Specific gels can allow larger dna and can be used for larger dna sorting
My big mystery, why do molecules get stuck? Counterions are moving in the opposite direction of the flow of dna. Eventually a balance is struck, the molecules stop, they can’t move forward anymore and the counterion force (and something else?) is holding them from moving.
My theory is that counterion flow must begin to make channels in the gel. I see theory from others that the DNA strands or macromolecules are being snaked through the gel, perhaps weaving through the gel fibers. That process could be making channels in the gel. Also channels in the gel could be made simply by counterion flow. These channels are the center of my theory.
My theory is based on channels happening in the gel, and it is that the channels in the gel are less intense near the electrode the molecules are moving away from, and near the electrode the molecules are moving towards, intense channels (high flow) of counterions move and prevent anything except the smallest molecules from migrating. Once a channel is made, the flow (of counterions) has less pressure, just like in a river where the smaller the river channel, the faster the flow.
You can put many different organic molecules in the gel and they will get sorted by size. A really neat machine! Below is the machine used for this experiment:
Below snip of Bruno Zimm document on electrophoresis, discussing counterions:
References:
Pulse-field gel
DNA analysis using analytical gels
DNA questions re counterion flow, Bruno Zimm document:
problems_and_prospects_in_the_theory_of_gel_electrophoresis_of_dna.pdf
Next learning is pcr, polymerase chain reaction. Using the famous
opyright 2026 Rod Deluhery
bacteria from Yellowstone national park, yellowstone geyser polymerase pcr.
yellowstone geyser polymerase pcr +9 Thermus aquaticus ((Taq)), a bacterium discovered in Yellowstone’s Mushroom Spring in 1966 by Thomas Brock
+9
Thermus aquaticus (\(Taq\)), a bacterium discovered in Yellowstone’s Mushroom Spring in 1966 by Thomas Brock
Three patients diagnosed with hereditary spherocytosis caused by different mutations (panels A–C) showed a spherocyte count of 11% (A), 8% (B), and 10% (C) in their stained peripheral blood smears, as exemplified in panel A (arrows, objective-magnification 100x).
so, be interesting. I can’t come to any conclusions yet, but I don’t see the same things that these people are seeing in terms of percentages of non disk blood cells.
I’m not a researcher by trade. I’m a researcher by hobbyist, but I just see that some of these researchers do not put in enough detail in how they came to the facts are the data that they’re showing. In this one, they don’t talk about how they got the micrograph pictures or the microscope pictures that they show. If they just took blood cells and smeared them on a slide, they would quickly dry up and they would form into different shapes. If you’re trying to figure out the sizes and shapes of blood cells, why would you do it that way?Why not use the immersion microscopy? Some of this research just is just I hate to say it. But it just seems to be like some of it is just garbage where somebody was trying to impress somebody or something. I mean, I don’t try to impress anybody. I’m just trying to get facts. I show facts, and then I show what I do, and then people can jump to whatever conclusions they want. But you have to tell in science, you have to tell everything about how you came to the data or to the photos or to the graph.You have to explain exactly how you got there. It doesn’t contribute much to science.If you don’t explain every little part of how you got there. Thanks for the researchers that do this and put in all the detailed work on how they got to their conclusions.
i have to do more work.But so far it does not add up.
in a previous post, I was fascinated by the components of human blood, and how they look nothing like most illustrations of blood. I did these photos with immersion microscopy, yet I did not use oil immersion. Instead, I simply put the microscope lens directly into the specimen of water blood mixture, so there was no air refraction. What I am still curious about is the spherical shape of the blood. I need to do more tests with hypertonic and hypotonic solutions to confirm the shape of my fingertip blood.
Original post about looking using immersion microscopy, mysteries of blood:
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